Unknown organism report

The MSA will select for organisms such as Staphylococcus species which can live in areas of high salt concentration plate on the left in the picture below. This is in contrast to Streptococcus species, whose growth is selected against by this high salt agar plate on the right in the picture below. The differential ingredient in MSA is the sugar mannitol.

Unknown organism report

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Metera Lab Report 3: Labs 7 and 8- Metabolism and Biochemical Tests Abstract This experiment focused on metabolism and biochemical tests. The goal of performing these tests was to differentiate microbes from one another and to compare how metabolic and biochemical processes differ from species to species.

The microbes that were tested during this lab were: Escherichia coli, Bacillus cereus, the unknown, Proteus vulgaris, Staphylococcus epidermis, Enterobacter aerogenes, the control, and Pseudomonas fluorescens.

The microbes tested during these various tests were looking for which would: Introduction The purpose of these labs was to observe various metabolic processes by determining the pH of certain bacteria, determining if the bacteria was urease positive or negative, determining which bacteria ferment which sugar s during fermentation, and determining if bacteria are lactose fermenters and non-lactose fermenters.

Identifying Bacteria

The tests performed to determine these metabolic processes include: The bacteria tested include: The different types of microbes studied in this experiment include: Escherichia coli, Bacillus cereus, Proteus vulgaris, Staphylococcus epidermis, Enterobacter aerogenes, and Pseudomonas fluorescens.

Escherichia coli is mainly found in animal feces and comprises their intestines as well US Food and Drug Administration. Bacillus cereus is a known medium of food poisoning and causes vomiting and abdominal cramps Todar.

Proteus vulgaris is connected with food spoilage of meat, poultry, and seafood and may cause diarrhea in infants Schenectady Country Community College. Staphylococcus epidermis often infects hospital patients with weak immune systems in catheter wounds European Bioinformatics Institute. Enterobacter aerogenes is the source of numerous infections such as bacteremia, lower respiratory tract infections, skin and soft tissue infections, urinary tract infections UTIsendocarditis, intra-abdominal infections, septic arthritis, osteomyelitis, and ophthalmic infections E Medicine.

Pseudomonas fluorescens are able to grow in various conditions such as soil, water, and plant habitats European Bioinformatics Institute. Several hypotheses arise during this experiment due to the many subjects being tested. However, since there are numerous tests being performed, a more general hypothesis can be ascertained.

The hypothesis for all tests in both Lab 7 and Lab 8 is that the outcome of the tests will produce the desired results in order to differentiate various species of bacteria from one another and to reveal certain characteristics of metabolic and biochemical processes.

Materials and Methods Lab 7 For Part A of Lab 7, label Escherichia coli, Proteus vulgaris, the unknown, and Enterobacter aerogenes on a blue sucrosea green glucoseand a red lactose tube. Then, using aseptic technique, inoculate each bacteria into each color tube by sticking the inoculating loop to the bottom of the tube and twirling it, then pulling it straight out.

Using aseptic technique, inoculate each tube with the corresponding bacteria by streaking the surface of the agar slant. Using aseptic technique, inoculate the labeled parts of the plate. Then, add ten drops of sulfanilic acid anddemehtylnapthylamine. If a red color develops after this step, record the record the results.

If not, add zinc dust to the tube and vortex it. For Part C, label Enterobacter aerogenes and Bacillus cereus on the milk agar plate.

Using aseptic technique, inoculate the plate with the corresponding bacteria. For Part D, put a few drops of water on the slide and then inoculate it with Bacillus cereus.

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Next, add one drop of hydrogen peroxide to the sample. For Part E, use a sterile swab to transfer the cells from Enterobacter aerogenes and Pseudomonas fluorescens to a disk.

Use a new swab for each sample. Add one drop of water to each disk. Part A [pic] [pic] Figure 1 Figure 2 Figure 1 is the unknown for sucrose. As shown, it had an orange Figure 2 is Escherichia coli for sucrose.


As shown, it was ring at the top that fades to yellow at the bottom, was cloudy orange throughout, had darker solution inside the tube than out, all the way through, and had no bubbles.

As shown, it was Figure 4 is Bacillus cereus for sucrose. As shown, it had a dark yellow and cloudy throughout, and had no bubbles. As shown, it was Figure 6 is the unknown for glucose. As shown, it was Figure 8 is Bacillus cereus for glucose. As shown, it was orange yellow, cloudy at the top, and had no bubbles.

As shown, it was uniformly Figure 10 is Enterobacter aerogenes for lactose.The Gram stain is a type of differential stain that allows a (micro)biologist to identify the differences between organisms and/or differences within the same organism.

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Jacket states: Special Limited Edition. Microbiology Unknown Project - Final Unknowns Report. You must use the Unknown Excel Template to develop a separate.

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report for each of your unknown bacteria. The template can be found linked to the online class syllabus. unknown lab report for microbiology An unknown bacterium was handed out by the lab instructor.

Unknown organism report

The methods that have been learned so far in identifying bacteria were applied to this unknown. Current: Microbiology Unknown Lab Report Microbiology Unknown Lab Report Upon obtaining the unknown organism, it was important to make a streak plate of the bacteria on TSA.

The purpose of doing so ensures that we have pure cultures of the unknown to be used in further testing and not a mixed culture.

Unknown organism, C 14 appeared pink in color following the Gram stain experiment, proving it is a Gram negative bacterium. This finding helped to eliminate the Gram positive. Kimsey Cooper Microbiology Lab Final Unknown Report Section#/ Radha Report on Identification of Mixed Culture Unknown. Lab Exercise 33 Introduction The purpose of this experiment is to apply the knowledge gained from the entire semester in the Microbiology Lab and apply it to be able to identify bacteria. Unknown Lab Report. Unknown Number Joel Hentrich. April 29 th, Microbiology. Spring INTRODUCTION The purpose of this study was multifaceted: First, it was completed in order to gain a better understanding of how to utilize microbiological techniques learned within the classroom and laboratory environment.

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